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Effects of low‐dose aspirin on gastric erosions, cyclooxygenase expression and mucosal prostaglandin‐E 2 do not depend on Helicobacter pylori infection
Author(s) -
VENERITO M.,
TREIBER G.,
WEX T.,
KUESTER D.,
ROESSNER A.,
DI MARIO F.,
MALFERTHEINER P.
Publication year - 2006
Publication title -
alimentary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.308
H-Index - 177
eISSN - 1365-2036
pISSN - 0269-2813
DOI - 10.1111/j.1365-2036.2006.02856.x
Subject(s) - medicine , prostaglandin e2 , aspirin , helicobacter pylori , cyclooxygenase , helicobacter pylori infection , gastroenterology , prostaglandin , prostaglandin e , biology , enzyme , biochemistry
Summary Background The mechanisms by which Helicobacter pylori and low‐dose aspirin induce gastric damage are not completely elucidated. Aim To evaluate the effects of low‐dose aspirin on gastric damage, mucosal prostaglandin‐E 2 levels and cyclooxygenase‐enzyme expression in relation to the H. pylori status. Methods Twenty healthy volunteers ( H. pylori positive, n = 10; H. pylori negative, n = 10) received aspirin 100 mg/die for 1 week. At days 0, 1, 3 and 7, gastric mucosal lesions were studied by oesophagogastroduodenoscopy and histology. COX‐1 and COX‐2 were determined by immunohistochemistry and reverse‐transcriptase polymerase chain reaction, and mucosal prostaglandin‐E 2 levels by enzyme‐linked immunosorbent assay. Nine H. pylori ‐positive subjects repeated the protocol after H. pylori eradication. Results All groups developed a similar number of erosions. COX‐1 and COX‐2 expression, as well as mucosal prostaglandin‐E 2 levels were not influenced by H. pylori status and aspirin medication. Helicobacter pylori ‐negative and H. pylori ‐eradicated subjects who developed aspirin‐induced erosions had significant lower pre‐treatment antral prostaglandin‐E 2 levels than those without erosions (3.6 ng/ μ g vs. 6.3 ng/ μ g protein and 3.6 ng/ μ g vs. 6.0 ng/ μ g protein, respectively, P < 0.01 Mann–Whitney U ‐test). Conclusions In healthy subjects, low‐dose aspirin for 1 week does neither affect cyclooxygenase expression nor mucosal prostaglandin‐E 2 levels. Antral prostaglandin‐E 2 ‐basal levels appear to be critical for development of aspirin‐induced gastric damage in subjects without H. pylori infection.