Open AccessHuman T Cell Leukemia Cell Death by Apoptosis‐inducing Nucleosides from CD57 + HLA‐DR bright Natural Suppressor Cell Line
Author(s) -
Mori Tsuneatsu,
Li Xiang,
Mori Etsuko,
Guo Maowu
Publication year - 2000
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.2000.tb00992.x
Subject(s) - apoptosis , programmed cell death , cell culture , leukemia , cell growth , cell , suppressor , microbiology and biotechnology , biology , cancer research , chemistry , immunology , biochemistry , cancer , genetics
Apoptosis‐inducing nucleosides (AINs) released from CD57 + HLA‐DR bright natural suppressor (57.DR‐NS) cell line, derived from human decidual tissue, were isolated from 57.DR‐NS cell culture supernatant by the combination of thin‐layer chromatography (TLC) and high‐performance liquid chromatography (HPLC). Apoptotic cell death was strongly induced in human T cell leukemia Molt4 cells treated with AINs, absolutely depending on DNA strand breaks, with activation of the caspase cascade, especially caspase‐3. The administration of AINs to Molt4 tumor‐bearing severe combined immunodeficiency (SCID) mice resulted in drastic suppression of tumor growth, with a decrease of tumor size and the appearance of apoptotic signals in tumor tissue. Thus, AINs are candidates for development as anticancer agents.