Premium
Cloning and Sequencing of the Gene Encoding the Candidate HtrA of Rickettsia typhi
Author(s) -
Kim Jonghyun,
Hahn Myongjoon
Publication year - 2000
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2000.tb02495.x
Subject(s) - biology , recombinant dna , gene , virology , clone (java method) , cloning (programming) , antiserum , signal peptide , microbiology and biotechnology , peptide sequence , antibody , genetics , computer science , programming language
We screened a phage library of Rickettsia typhi with a polyclonal antiserum to clone genes which encode immunogenic proteins of R. typhi . Among several clones obtained, one clone codes for a 466‐amino‐acid protein similar to the heat‐shock protein, HtrA. The deduced rickettsial HtrA contains a putative signal peptide sequence at the N‐terminus, a serine protease‐like domain, and two PDZ domains. The recombinant protein of rickettsial HtrA reacted with sera from patients with murine typhus and tsutsugamushi disease. We suggest that this gene and its recombinant protein would be valuable for the immunologic diagnosis of rickettsial diseases.