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Water‐based measurement of rainbow trout melatonin
Author(s) -
James J. D.,
Ellis T.,
Scott A. P.
Publication year - 2004
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.0022-1112.2004.00531.x
Subject(s) - rainbow trout , melatonin , biology , radioimmunoassay , trout , zoology , fish <actinopterygii> , fishery , endocrinology
Melatonin (MLT) was isolated from water samples using solid phase extraction cartridges and measured by radioimmunoassay. In tanks of rainbow trout Oncorhynchus mykiss , concentrations of MLT in water increased rapidly from <0·1 ng l −1 in the light phase to 0·7 ng l −1 in the dark giving calculated release rates of <1 ng kg −1 h −1 and 15 ng kg −1 h −1 , respectively. This cycle in water MLT values corresponds to reported changes in plasma MLT concentrations. The colour of the tanks and a daytime acute handling stress did not affect concentrations of MLT in either the light or dark phase.