English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

Laodelphax striatellus  is a sap‐feeding pest and the main insect vector of  rice stripe virus  (RSV). There is an urgent need to identify molecular targets to control this insect pest and plant arboviruses. In this study, we identified a  L. striatellus  gene (named  LsGrpE ) encoding a GroP‐E‐like protein. We found that the LsGrpE protein localized to mitochondria. Using gene‐specific dsRNA to interfere with the expression of  LsGrpE  led to a significant increase in insect mortality, and most of the surviving insects could not develop into adults. Further analyses revealed that  LsGrpE  deficiency caused mitochondrial dysfunction and inhibited the insulin pathway, resulting in diabetes‐like symptoms such as elevated blood sugar, inactive behaviour, developmental delay, and death. In addition,  LsGrpE  deficiency significantly reduced the RSV titre in surviving  L. striatellus , and indirectly prevented viral vertical transmission by reducing the number of adults. We generated transgenic rice plants expressing  LsGrpE ‐specific dsRNA, and the dsRNA was acquired by  L. striatellus  during feeding, resulting in increased insect mortality and the prevention of arboviral transmission. This study clarifies the function of LsGrpE and demonstrates that LsGrpE can be used as a molecular target of plant‐generated dsRNA to resist this sap‐feeding pest, a17nd therefore, its transmitted arboviruses.

GrpE is involved in mitochondrial function and is an effective target for RNAi ‐mediated pest and arbovirus control