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A transferrin gene associated with development and 2‐tridecanone tolerance in H elicoverpa armigera
Author(s) -
Zhang L.,
Shang Q.,
Lu Y.,
Zhao Q,
Gao X.
Publication year - 2015
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/imb.12129
Subject(s) - helicoverpa armigera , biology , rna silencing , rna interference , microbiology and biotechnology , gene , complementary dna , malpighian tubule system , transferrin , rna , green fluorescent protein , epidermis (zoology) , messenger rna , genetics , larva , botany , biochemistry , anatomy , midgut
The full‐length c DNA (2320 bp) encoding a putative iron‐binding transferrin protein from H elicoverpa armigera was cloned and named H a T rf . The putative H a T rf sequence included 670 amino acids with a molecular mass of approximately 76 kDa. Quantitative PCR results demonstrated that the transcriptional level of H a T rf was significantly higher in the sixth instar and pupa stages as compared with other developmental stages. H a T rf transcripts were more abundant in fat bodies and in the epidermis than in malpighian tubules. Compared with the control, the expression of H a T rf increased dramatically 24 h after treatment with 2‐tridecanone. Apparent growth inhibition with a dramatic body weight decrease was observed in larvae fed with H a T rf double‐stranded RNA (ds RNA ), as compared with those fed with green fluorescent protein ds RNA . RNA interference of H a T rf also significantly increased the susceptibility of larvae to 2‐tridecanone. These results indicate the possible involvement of H a T rf in tolerance to plant secondary chemicals.