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Disease characteristics of bovine spongiform encephalopathy following inoculation into mice via three different routes
Author(s) -
Vickery Christopher M.,
Beck Katy E.,
Simmons Marion M.,
Hawkins Stephen A. C.,
Spiropoulos John
Publication year - 2013
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1111/iep.12036
Subject(s) - infectivity , inoculation , bovine spongiform encephalopathy , lesion , virology , strain (injury) , incubation period , biology , mesenteric lymph nodes , incubation , medicine , pathology , disease , spleen , immunology , anatomy , virus , prion protein , biochemistry
Summary Mouse‐adapted transmissible spongiform encephalopathy ( TSE ) strains are routinely distinguished based on reproducible disease characteristics in a given mouse line following inoculation via a consistent route. We investigated whether different administration routes (oral, intragastric (i.g.) and intracerebral (i.c.)) can alter the disease characteristics in IM mice after serial dilution of a stabilized mouse‐adapted bovine spongiform encephalopathy ( BSE ) strain (301 V ). In addition, the infectivity of distal ileum and mesenteric lymph nodes (ln) sampled at three time points (35 days postinoculation (dpi), 70 dpi and terminal disease) after i.g. inoculation of 301 V strain was assessed in mice by i.c. challenge. Strain characteristics were assessed according to standard methodology and P r P Sc immunohistochemistry deposition patterns. Mean incubation periods were prolonged following oral or i.g. inoculations compared to the i.c. route. Lesion profiles following i.c. challenges were elevated compared to i.g. and oral routes although vacuolation in the dorsal medulla was consistently high irrespective of the route of administration. Nevertheless, the same P r P Sc deposition pattern was associated with each route of administration. Distal and mesenteric ln infectivity was detected as early as 35 dpi and displayed consistent lesion profiles and P r P Sc deposition patterns. Our data suggest that although 301 V retained its properties, some phenotypic parameters were affected by the route of inoculation. We conclude that bioassay data should be interpreted carefully and should be standardized for route of inoculation.