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Experimental rat model for alcohol‐induced osteonecrosis of the femoral head
Author(s) -
Okazaki Shunichiro,
Nagoya Satoshi,
Tateda Kenji,
Katada Ryuichi,
Mizuo Keisuke,
Watanabe Satoshi,
Yamashita Toshihiko,
Matsumoto Hiroshi
Publication year - 2013
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1111/iep.12035
Subject(s) - pathogenesis , steatosis , femoral head , medicine , alcoholic liver disease , alcohol , fatty liver , liquid diet , tlr4 , ethanol , alcoholic fatty liver , endocrinology , disease , receptor , cirrhosis , chemistry , surgery , biochemistry
Summary Alcohol‐induced osteonecrosis of the femoral head ( ONFH ) is observed in alcohol abusers and patients with alcoholic fatty liver disease. It has been reported that Toll‐like receptor 4 ( TLR 4) signalling plays a crucial role in the pathogenesis of alcoholic fatty liver disease. We previously reported a corticosteroid‐induced ONFH rat model, and suggested that TLR 4 signalling contributes to the pathogenesis of ONFH . Thus, it is thought that the pathogenesis of alcohol‐induced ONFH is probably similar to that of corticosteroid‐induced ONFH . The aim of this study was to develop a new animal model for alcohol‐induced ONFH and to evaluate the relationship between the pro‐inflammatory response via TLRs and the development of ONFH in rats. Male Wistar rats were fed a Lieber–DeCarli liquid diet containing 5% ethanol (experimental group) or dextran (control group) for 1–24 weeks. Histopathological and biochemical analyses were performed. Feeding the ethanol‐containing liquid diet resulted in the development of ONFH with hepatic steatosis, hepatic dysfunction and hyperlipidaemia, whereas feeding the dextran‐containing diet did not cause ONFH . However, we could not recognize any relationship between the pro‐inflammatory response via TLR 4 and the development of alcohol‐induced ONFH . Thus in this study we have developed a new rat model for alcohol‐induced ONFH based on the feeding of an ethanol liquid diet. ONFH was observed within seven days from the start of feeding with 5% ethanol‐containing liquid diet. Although this was linked to hepatic steatosis, a TLR4 association was not a feature of this model.