English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

Aims  Pulmonary large cell neuroendocrine carcinoma ( LCNEC ) is underdiagnosed on biopsy specimens. We evaluated if routine neuroendocrine immunohistochemical ( IHC ) stains are helpful in the diagnosis of  LCNEC  on biopsy specimens.    Methods and results  Using the Dutch pathology registry ( PALGA ), surgically resected  LCNEC  with matching pre‐operative biopsy specimens were identified and haematoxylin and  IHC  slides ( CD 56, chromogranin‐A, synaptophysin) requested. Subsequently, three pathologists assigned (1) the presence or absence of the  WHO  2015 criteria and (2) cumulative size of all (biopsy) specimens. For validation, a tissue microarray ( TMA ) of non‐small‐cell lung cancer ( NSCLC ) ( n  =   77) and  LCNEC  ( n  =   19) was used.  LCNEC  was confirmed on the resection specimens in 32 of 48 re‐reviewed cases. In 47% ( n  = 15 of 32)  LCNEC  was also confirmed in the paired biopsy specimens. Neuroendocrine morphology was absent in 53% ( n  = 17 of 32) of paired biopsy specimens, more often when smaller amounts of tissue were available for evaluation [29% < 5 mm ( n  = 14) versus 67% ≥5 mm ( n  = 18)  P  = 0.04]. Combined with current  WHO  criteria, positive staining for greater than or equal to two of three neuroendocrine  IHC  markers increased the sensitivity for  LCNEC  from 47% to 93% on paired biopsy specimens, and further validated using an independent  TMA  of  LCNEC  and  NSCLC  with sensitivity and specificity of 80% and 99%, respectively.    Conclusions   LCNEC  is difficult to diagnose because neuroendocrine morphology is frequently absent in biopsy specimens. In  NSCLC  devoid of obvious morphological squamous or adenocarcinoma features, positive staining in greater than or equal to two of three neuroendocrine  IHC  stains supports the diagnosis of  LCNEC .

histopathology

Is the sum of positive neuroendocrine immunohistochemical stains useful for diagnosis of large cell neuroendocrine carcinoma (LCNEC) on biopsy specimens?