English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

Aims  To check the usefulness of a standardized protocol of   PTEN    FISH  in 31 endometrial carcinomas ( EC s) in comparison with  SNP  array ( SNPA ), multiplex ligation‐dependent probe amplification ( MLPA ), and immunohistochemistry.    Methods and results  Fluorescence  in‐situ  hybridization analysis showed two   PTEN   copies in 17 cases, three copies in nine cases, hemizygous deletion in two cases, and diverse cell populations with different   PTEN   copy number in three cases. A good correlation was seen between  FISH  and  SNPA , particularly in cases with three copies.  FISH  identified two cases with entire deletion of chromosome 10, but did not identify a focal deletion of   PTEN  . Five cases with   PTEN   deletion and duplication of the second allele by  SNPA  were interpreted as normal by  FISH . Concordance between  FISH  and  MLPA  was seen in 15 cases with two copies, and in two cases with   PTEN   deletion. Six cases were interpreted as amplified by  MLPA , but showed polyploidy by  FISH .  FISH  was superior to  SNPA  and  MLPA  in assessing the tumours with diverse cell populations with different   PTEN   copies.    Conclusions  The results show good concordance between  FISH ,  SNPA  and  MLPA .  SNPA  was superior in tumours with deletion of one copy and duplication of the second allele.  FISH  was superior in assessing tumour heterogeneity.

FISH analysis of PTEN in endometrial carcinoma. comparison with SNP arrays and MLPA