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Using unnatural amino acids to selectively label proteins for cellular imaging: a cell biologist viewpoint
Author(s) -
Elia Natalie
Publication year - 2021
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.15477
Subject(s) - green fluorescent protein , fluorescent protein , chemical biology , computational biology , förster resonance energy transfer , amino acid , fluorescence , cell , biology , chemistry , biochemistry , microbiology and biotechnology , gene , physics , quantum mechanics
This viewpoint provides an overview of applying genetic code expansion and click chemistry for labeling proteins for cellular imaging applications. This approach, which enables labeling of proteins in cells at a specific site with fluorescent dyes, has several advantages for cellular imaging. However, it also introduces limitations and challenges for cellular imaging. The current state of the field is discussed as well as the advantages and limitations of this unique approach.