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Dihydroavenanthramide D prevents UV ‐irradiated generation of reactive oxygen species and expression of matrix metalloproteinase‐1 and ‐3 in human dermal fibroblasts
Author(s) -
Kim JeongMi,
Noh EunMi,
Kwon KangBeom,
Hwang BoMi,
Hwang JinKi,
You YongOuk,
Kim Min Seuk,
Lee Wan,
Lee JeongHo,
Kim HyeJung,
Kim JongSuk,
Lee YoungRae
Publication year - 2013
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12243
Subject(s) - matrix metalloproteinase , reactive oxygen species , chemistry , microbiology and biotechnology , metalloproteinase , matrix (chemical analysis) , biochemistry , biology , chromatography
Ultraviolet B ( UVB ) radiation induces photoageing by upregulating the expression of matrix metalloproteinases ( MMP s) in human skin cells. D ihydroavenanthramide D ( DHA vD) is a synthetic analog to naturally occurring avenanthramide, which is the active component in oats. Although anti‐inflammatory, anti‐atherosclerotic and antioxidant effects have been reported, the antiphotoageing effects of DHA v D are yet to be understood. In this study, we investigated the inhibitory effects of DHA v D on UVB ‐induced production of reactive oxygen species ( ROS ) and expression of MMP s, and its molecular mechanism in UVB ‐irradiated human dermal fibroblasts. Western blot and real‐time PCR analyses revealed that DHA v D inhibited UVB ‐induced MMP ‐1 and MMP ‐3 expression. It also significantly blocked UVB ‐induced ROS generation in fibroblasts. Additionally, DHA v D attenuated UVB ‐induced phosphorylation of MAPK s, activation of NF ‐κB and AP ‐1. DHA v D regulates UVB ‐irradiated MMP expression by inhibiting ROS ‐mediated MAPK / NF ‐κB and AP ‐1 activation. DHA v D may be a useful candidate for preventing UV light‐induced skin photoageing.