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Summary   Background  Inertial measurement unit ( IMU ) sensor‐based techniques are becoming more popular in horses as a tool for objective locomotor assessment.    Objectives  To describe, evaluate and validate a method of stride detection and quantification at walk and trot using distal limb mounted  IMU  sensors.    Study design  Prospective validation study comparing  IMU  sensors and motion capture with force plate data.    Methods  A total of seven Warmblood horses equipped with metacarpal/metatarsal  IMU  sensors and reflective markers for motion capture were hand walked and trotted over a force plate. Using four custom built algorithms hoof‐on/hoof‐off timing over the force plate were calculated for each trial from the  IMU  data. Accuracy of the computed parameters was calculated as the mean difference in milliseconds between the  IMU  or motion capture generated data and the data from the force plate, precision as the s.d. of these differences and percentage of error with accuracy of the calculated parameter as a percentage of the force plate stance duration.    Results  Accuracy, precision and percentage of error of the best performing  IMU  algorithm for stance duration at walk were 28.5, 31.6 ms and 3.7% for the forelimbs and −5.5, 20.1 ms and −0.8% for the hindlimbs, respectively. At trot the best performing algorithm achieved accuracy, precision and percentage of error of −27.6/8.8 ms/−8.4% for the forelimbs and 6.3/33.5 ms/9.1% for the hindlimbs.    Main limitations  The described algorithms have not been assessed on different surfaces.    Conclusions  Inertial measurement unit technology can be used to determine temporal kinematic stride variables at walk and trot justifying its use in gait and performance analysis. However, precision of the method may not be sufficient to detect all possible lameness‐related changes. These data seem promising enough to warrant further research to evaluate whether this approach will be useful for appraising the majority of clinically relevant gait changes encountered in practice.

Validation of distal limb mounted inertial measurement unit sensors for stride detection in Warmblood horses at walk and trot