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Role of G o/i subgroup of G proteins in olfactory signaling of D rosophila melanogaster
Author(s) -
Ignatious Raja Jennifer S.,
Katanayeva Natalya,
Katanaev Vladimir L.,
Galizia C. Giovanni
Publication year - 2014
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/ejn.12481
Subject(s) - heterotrimeric g protein , pertussis toxin , metabotropic receptor , microbiology and biotechnology , calcium imaging , metabotropic glutamate receptor , g protein , biology , chemistry , signal transduction , neuroscience , receptor , calcium , biochemistry , glutamate receptor , organic chemistry
Intracellular signaling in insect olfactory receptor neurons remains unclear, with both metabotropic and ionotropic components being discussed. Here, we investigated the role of heterotrimeric G o and G i proteins using a combined behavioral, in vivo and in vitro approach. Specifically, we show that inhibiting G o in sensory neurons by pertussis toxin leads to behavioral deficits. We heterologously expressed the olfactory receptor dO r22a in human embryonic kidney cells (HEK293T). Stimulation with an odor led to calcium influx, which was amplified via calcium release from intracellular stores. Subsequent experiments indicated that the signaling was mediated by the G βγ subunits of the heterotrimeric G o/i proteins. Finally, using in vivo calcium imaging, we show that G o and G i contribute to odor responses both for the fast (phasic) as for the slow (tonic) response component. We propose a transduction cascade model involving several parallel processes, in which the metabotropic component is activated by G o and G i , and uses G βγ.