English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

Background and Aim  Endocytoscopy ( EC ) at ultra‐high magnification enables   in vivo   visualization of cellular atypia of gastrointestinal mucosae. Clear images are essential for precise diagnosis by  EC . The aim of the present study was to evaluate the optimal staining method for  EC  in the colon.    Methods  Thirty prospectively enrolled patients were allocated 1:1:1 to three distinct staining methods: 0.05% crystal violet ( CV ) alone, 1% methylene blue ( MB ) alone, or  CV  +  MB  ( CM  double). Normal rectal mucosae were stained with each dye and videos of  EC  images were recorded. Visibility of nuclei and gland formation after staining were evaluated as ‘recognizable’ or ‘not recognizable’. Time for each parameter to become ‘recognizable’ was measured, and the average times for the three staining regimens were compared.    Results   MB  alone and  CM  double staining resulted in ‘recognizable’ (102 ± 27  vs  89 ± 22 s,  P  = 0.263) nuclei within comparable periods of time, whereas  CV  alone was unable to identify nuclei. Gland formation became ‘recognizable’ sooner after  CM  double staining than after  MB  alone (61 ± 16  vs  108 ± 24 s,  P  < 0.001).    Conclusions  Double staining with  CV  and  MB , which rapidly provided recognizable images of both nuclei and gland formation, is an appropriate staining regimen for colonic  EC .

Double staining with crystal violet and methylene blue is appropriate for colonic endocytoscopy: A n in vivo prospective pilot study