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Double staining with crystal violet and methylene blue is appropriate for colonic endocytoscopy: A n in vivo prospective pilot study
Author(s) -
Ichimasa Katsuro,
Kudo Shinei,
Mori Yuichi,
Wakamura Kunihiko,
Ikehara Nobunao,
Kutsukawa Makoto,
Takeda Kenichi,
Misawa Masashi,
Kudo Toyoki,
Miyachi Hideyuki,
Yamamura Fuyuhiko,
Ohkoshi Shogo,
Hamatani Shigeharu,
Inoue Haruhiro
Publication year - 2014
Publication title -
digestive endoscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.5
H-Index - 56
eISSN - 1443-1661
pISSN - 0915-5635
DOI - 10.1111/den.12164
Subject(s) - staining , crystal violet , in vivo , pathology , medicine , methylene blue , atypia , microbiology and biotechnology , nuclear medicine , chemistry , biology , biochemistry , photocatalysis , catalysis
Background and Aim Endocytoscopy ( EC ) at ultra‐high magnification enables in vivo visualization of cellular atypia of gastrointestinal mucosae. Clear images are essential for precise diagnosis by EC . The aim of the present study was to evaluate the optimal staining method for EC in the colon. Methods Thirty prospectively enrolled patients were allocated 1:1:1 to three distinct staining methods: 0.05% crystal violet ( CV ) alone, 1% methylene blue ( MB ) alone, or CV + MB ( CM double). Normal rectal mucosae were stained with each dye and videos of EC images were recorded. Visibility of nuclei and gland formation after staining were evaluated as ‘recognizable’ or ‘not recognizable’. Time for each parameter to become ‘recognizable’ was measured, and the average times for the three staining regimens were compared. Results MB alone and CM double staining resulted in ‘recognizable’ (102 ± 27 vs 89 ± 22 s, P = 0.263) nuclei within comparable periods of time, whereas CV alone was unable to identify nuclei. Gland formation became ‘recognizable’ sooner after CM double staining than after MB alone (61 ± 16 vs 108 ± 24 s, P < 0.001). Conclusions Double staining with CV and MB , which rapidly provided recognizable images of both nuclei and gland formation, is an appropriate staining regimen for colonic EC .