Open Access10 −7 m 17β‐oestradiol enhances odonto/osteogenic potency of human dental pulp stem cells by activation of the NF‐κB pathway
Author(s) -
Wang Y.,
Zheng Y.,
Wang Z.,
Li J.,
Wang Z.,
Zhang G.,
Yu J.
Publication year - 2013
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12071
Subject(s) - runx2 , dental pulp stem cells , alkaline phosphatase , chemistry , mesenchymal stem cell , western blot , downregulation and upregulation , microbiology and biotechnology , potency , stem cell , signal transduction , osteoblast , biology , biochemistry , in vitro , enzyme , gene
Objectives Oestrogen has been proven to significantly enhance osteogenic potency, while oestrogen deficiency usually leads to impaired osteogenic differentiation of mesenchymal stem cells. However, little is known concerning direct effects of oestrogen on differentiation of human dental pulp stem cells ( DPSC s). Materials and methods In this study, human DPSC s were isolated and treated with 10 −7 m 17β‐oestradiol (E2). Alkaline phosphatase ( ALP ) assay and alizarin red staining were performed. Results Alkaline phosphatase and alizarin red showed that E2 treatment significantly enhanced ALP activity and mineralization ability of DPSC s, but had no effect on cell proliferation. Real‐time RT ‐ PCR and western blot assay demonstrated that odonto/osteogenic markers ( ALP , RUNX2 /RUNX2, OSX /OSX, OCN /OCN and DSPP /DSP) were significantly upregulated in the cells after E2 treatment. Moreover, phosphorylation of cytoplasmic IκBα/P65 and expression of nuclear P65 were enhanced in a time‐dependent manner following E2 treatment, suggesting activation of NF‐κB signaling. Conversely, inhibition of the NF‐κB pathway suppressed E2‐mediated upregulation of odonto/osteogenic markers, indicating that the NF‐κB pathway was pivotal for E2‐mediated differentiation. Conclusion These findings provide evidence that 10 −7 m 17β‐oestradiol promoted odonto/osteogenic differentiation of human DPSC s via activation of the NF‐κB signaling pathway.