Open AccessDecreased expression of T‐cell‐associated immune markers predicts poor prognosis in patients with follicular lymphoma
Author(s) -
Rai Shinya,
Inoue Hiroaki,
Sakai Kazuko,
Hanamoto Hitoshi,
Matsuda Mitsuhiro,
Maeda Yasuhiro,
Haeno Takahiro,
Watatani Yosaku,
Kumode Takahiro,
Serizawa Kentaro,
Taniguchi Yasuhiro,
Hirase Chikara,
Espinoza J. Luis,
Morita Yasuyoshi,
Tanaka Hirokazu,
Ashida Takashi,
Tatsumi Yoichi,
Nishio Kazuto,
Matsumura Itaru
Publication year - 2022
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.15224
Subject(s) - follicular lymphoma , medicine , biology , oncology , progression free survival , cd8 , international prognostic index , lymphoma , diffuse large b cell lymphoma , immune system , bcl6 , germinal center , immunology , cancer research , b cell , chemotherapy , antibody
We previously examined the utility of rituximab‐bendamustine (RB) in patients with follicular lymphoma (FL) exhibiting less than optimal responses to 2 cycles of the R‐CHOP chemotherapy regimen. The aim of this study was to identify molecular biomarkers that can predict prognosis in RB‐treated patients in the context of the prospective cohort. We first analyzed the mutational status of 410 genes in diagnostic tumor specimens by target capture and Sanger sequencing. CREBBP , KMT2D , MEF2B , BCL2 , EZH2 , and CARD11 were recurrently mutated as reported before, however none was predictive for progression‐free survival (PFS) in the RB‐treated patients (n = 34). A gene expression analysis by nCounter including 800 genes associated with carcinogenesis and/or the immune response showed that expression levels of CD8 + T‐cell markers and half of the genes regulating Th1 and Th2 responses were significantly lower in progression of disease within the 24‐mo (POD24) group (n = 8) than in the no POD24 group (n = 31). Collectively, we selected 10 genes ( TBX21 , CXCR3 , CCR4 , CD8A , CD8B , GZMM , FLT3LG , CD3E , EOMES , GZMK ), and generated an immune infiltration score (IIS) for predicting PFS using principal component analysis, which dichotomized the RB‐treated patients into immune IIS high (n = 19) and IIS low (n = 20) groups. The 3‐y PFS rate was significantly lower in the IIS low group than in the IIS high group (50.0% [95% CI: 27.1‐69.2%] vs. 84.2% [95% CI: 58.7‐94.6%], P = .0237). Furthermore, the IIS was correlates with absolute lymphocyte counts at diagnosis ( r = 0.460, P = .00355). These results suggest that the T‐cell‐associated immune markers could be useful to predict prognosis in RB‐treated FL patients. (UMIN:000 013 795, jRCT:051 180 181)