Open AccessCharacteristics of B lymphocyte infiltration in HPV + head and neck squamous cell carcinoma
Author(s) -
Zhang Siwei,
Wang Bozhi,
Ma Fen,
Tong Fangjia,
Yan Bingqing,
Liu Tianyang,
Xie Huanhuan,
Song Lianhao,
Yu Siyang,
Wei Lanlan
Publication year - 2021
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.14834
Subject(s) - head and neck squamous cell carcinoma , cxcl13 , infiltration (hvac) , tumor infiltrating lymphocytes , b cell , lymphocyte , cancer research , cd38 , antibody , immunohistochemistry , medicine , pathology , chemokine , head and neck cancer , biology , cd8 , cancer , immunology , immune system , cd34 , stem cell , physics , genetics , chemokine receptor , thermodynamics
Human papillomavirus (HPV) is an important etiological factor of head and neck squamous cell carcinoma (HNSCC). HPV + HNSCC patients usually have a better prognosis, which probably results from the higher infiltration of B lymphocytes. This study was purposed to detect the infiltration of B lymphocyte subsets and the correlation between B lymphocyte subsets and the prognosis in HPV‐related HNSCC. In this study, 124 HPV + and 513 HPV − HNSCC samples were obtained from the Gene Expression Omnibus (GEO) database and The Cancer Genome Atlas (TCGA) database for transcriptomic analysis. Infiltration of B lymphocytes subsets was detected with 7 HPV + HNSCC and 13 HPV − HNSCC tissues through immunohistochemistry and immunofluorescence. One HPV − HNSCC sample was detected with single‐cell sequencing for chemokine analysis. In the results, the infiltration of plasma cells (CD19 + CD38 + ) and memory B cells (MS4A1 + CD27 + ) was higher in HPV + HNSCC samples. High infiltration of plasma cells and memory B cells was related to a better prognosis. High density of B lymphocytes was positively correlated with high CXCL13 production mainly from CD4 + T lymphocytes in HNSCC. These results indicated that a high density of plasma cells and memory B cells could predict excellent prognosis. CD4 + T lymphocytes might affect B lymphocytes and their subsets through the CXCL13/CXCR5 axis in HNSCC.