Open AccessCENP‐50 is required for papilloma development in the two‐stage skin carcinogenesis model
Author(s) -
Saito Megumi,
Kagawa Naoko,
Okumura Kazuhiro,
Munakata Haruka,
Isogai Eriko,
Fukagawa Tatsuo,
Wakabayashi Yuichi
Publication year - 2020
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.14533
Subject(s) - carcinogenesis , dmba , papilloma , cancer research , biology , stage (stratigraphy) , pathology , cancer , medicine , genetics , paleontology
Abstract CENP‐50/U is a component of the CENP‐O complex (CENP‐O/P/Q/R/U) and localizes to the centromere throughout the cell cycle. Aberrant expression of CENP‐50/U has been reported in many types of cancers. However, as Cenp‐50/U ‐deficient mice die during early embryogenesis, its functions remain poorly understood in vivo. To investigate the role of Cenp‐50/U in skin carcinogenesis, we generated Cenp‐50/U conditional knockout ( K14Cre ER ‐ Cenp‐50/U fl/fl ) mice and subjected them to the 7,12‐dimethylbenz(a)anthracene (DMBA)/terephthalic acid (TPA) chemical carcinogenesis protocol. As a result, early‐stage papillomas decreased in Cenp‐50/U ‐deficient mice. In contrast, Cenp‐50/U ‐deficient mice demonstrated almost the same carcinoma incidence as control mice. Furthermore, mRNA expression analysis using DMBA/TPA‐induced papillomas and carcinomas revealed that Cenp‐50/U expression levels in papillomas were significantly higher than in carcinomas. These results suggest that Cenp‐50/U functions mainly in early papilloma development and it has little effect on malignant conversion.