Open AccessUtility of isocitrate dehydrogenase 1 as a serum protein biomarker for the early detection of non‐small‐cell lung cancer: A multicenter in vitro diagnostic clinical trial
Author(s) -
Sun Nan,
Sun Shouguo,
Gao Yibo,
Li Yuan,
Lu Zhiliang,
Yuan Zuyang,
Che Yun,
Huang Jianbing,
Mao Shuangshuang,
Lei Yuanyuan,
Zang Ruochuan,
Li Ning,
Cui Wei,
Qi Jun,
Chen Feng,
Gao Jia,
Wang Jinling,
Min Rong,
Chen Yan,
Shi Guangli,
Tan Fengwei,
He Jie
Publication year - 2020
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.14387
Subject(s) - isocitrate dehydrogenase , medicine , lung cancer , cohort , biomarker , idh1 , lactate dehydrogenase , oncology , stage (stratigraphy) , cancer , receiver operating characteristic , gastroenterology , prospective cohort study , pathology , biology , enzyme , paleontology , biochemistry , gene , mutation
We aimed to verify the expression status and diagnostic significance of isocitrate dehydrogenase 1 (IDH1) in non‐small‐cell lung cancer (NSCLC), especially during early stages. Serum IDH1 levels were measured by ELISA. A total of 1223 participants (660 patients with NSCLC, 276 healthy controls [HCs], 95 patients with benign pulmonary conditions [BPCs], 135 patients with other cancers [OCs], and 57 samples with interfering factors) were divided into a training cohort and a validation cohort according to 3 testing centers. The IDH1 concentrations in the NSCLC group were obviously higher than those in the control groups ( P < .001). Area under the receiver operating characteristic curves (AUCs) for discriminating NSCLC patients from controls (HC, BPC, and OC) were 0.870 and 0.745 (sensitivity, 63.3% and 55.0%; specificity, 86.8% and 86.3%) in the training cohort and validation cohort, respectively. The AUCs for discriminating stage 0‐IA lung cancer patients from HCs were 0.907 and 0.788 (sensitivity, 58.6% and 59.1%; specificity, 92.9% and 89.3%) in 2 cohorts, respectively. Isocitrate dehydrogenase 1 showed specificity for NSCLC and had no diagnostic value for other common cancers. Furthermore, IDH1 was significantly reduced in postoperative serum. Isocitrate dehydrogenase 1 shows clinical utility as a serum protein biomarker for the early diagnosis of NSCLC.