English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

Chromosome 7q (Ch.7q) is clonally amplified in colorectal cancer (CRC). We aimed to identify oncogenes on Ch.7q that are overexpressed through DNA copy number amplification and determine the biological and clinical significance of these oncogenes in CRC. We identified general transcription factor 2I repeat domain‐containing protein 1 ( GTF2IRD1 ) as a potential oncogene using a CRC dataset from The Cancer Genome Atlas with a bioinformatics approach. We measured the expression of  GTF2IRD1  in 98 patients with CRC using immunohistochemistry and RT‐quantitative PCR (RT‐qPCR). The biological effects of  GTF2IRD1  expression were explored by gene set enrichment analysis (GSEA). Next, we undertook in vitro cell proliferation and cell cycle assays using si GTF2IRD1 ‐transfected CRC cells. We further investigated the oncogenic mechanisms through which  GTF2IRD1  promoted CRC progression. Finally, we assessed the clinical significance of  GTF2IRD1  expression by RT‐qPCR.  GTF2IRD1  was overexpressed in tumor cells and liver metastatic lesions. The GSEA revealed a positive correlation between  GTF2IRD1  expression and cell cycle progression‐related genes.  GTF2IRD1  knockdown inhibited cell proliferation and induced cell cycle arrest in  Smad4 ‐mutated CRC.  GTF2IRD1  downregulated the expression of the gene encoding transforming growth factor β receptor 2 ( TGFβR2 ), a tumor‐suppressor gene in  Smad4 ‐mutated CRC. On multivariate analysis, high  GTF2IRD1  expression was an independent poor prognostic factor. Clinicopathological analysis showed that  GTF2IRD1  expression was positively correlated with liver metastasis. In conclusion,  GTF2IRD1  promoted CRC progression by downregulating  TGFβR2  and could be a prognostic biomarker on Ch.7q in CRC.  GTF2IRD1  could also be a novel oncogene in CRC.

GTF2IRD1 on chromosome 7 is a novel oncogene regulating the tumor‐suppressor gene TGFβR2 in colorectal cancer