Open AccessNOP 2/Sun RNA methyltransferase 2 promotes tumor progression via its interacting partner RPL 6 in gallbladder carcinoma
Author(s) -
Gao Yuan,
Wang Zheng,
Zhu Yidi,
Zhu Qin,
Yang Yang,
Jin Yunpeng,
Zhang Fei,
Jiang Lin,
Ye Yuanyuan,
Li Huaifeng,
Zhang Yichi,
Liang Haibin,
Xiang Shanshan,
Miao Huijie,
Liu Yingbin,
Hao Yajuan
Publication year - 2019
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.14190
Subject(s) - cancer research , carcinogenesis , cell growth , methyltransferase , gene silencing , downregulation and upregulation , malignancy , biology , gallbladder , cancer , chemistry , medicine , methylation , biochemistry , genetics , gene
NOP 2/Sun domain family, member 2 ( NSUN 2) is a nuclear RNA methyl‐transferase catalyzing 5‐methylcytosine formation. Evidence shows that NSUN 2 is correlated with cell unlimited proliferation. However, its functional role in gallbladder carcinoma ( GBC ), which is the most common biliary tract malignancy and has a poor prognosis, remains to be determined. Here we found that NSUN 2 was highly expressed in GBC tissues as well as cell lines. NSUN 2 silencing repressed GBC cell proliferation and tumorigenesis both in vitro and in vivo. Conversely, upregulation of NSUN 2 enhanced GBC cell growth and colony formation. We further discovered that RPL 6 was a closely interacting partner with NSUN 2. Silencing RPL 6 resulted in insufficient NSUN 2 translational level and accumulative NSUN 2 transcriptional level. Exogenous expression of NSUN 2 partially rescued the effect of RPL 6 in gallbladder cancer progression. Taken together, our data provided novel mechanic insights into the function of NSUN 2 in GBC , thus pointing to NSUN 2 as a potential and effective therapeutic approach to GBC treatment.