Open AccessIdentification of new octamer transcription factor 1‐target genes upregulated in castration‐resistant prostate cancer
Author(s) -
Yamamoto Shinichiro,
Takayama Kenichi,
Obinata Daisuke,
Fujiwara Kyoko,
Ashikari Daisaku,
Takahashi Satoru,
Inoue Satoshi
Publication year - 2019
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.14183
Subject(s) - prostate cancer , gene knockdown , cancer research , transcription factor , biology , androgen receptor , gene , cancer , downregulation and upregulation , cancer cell , protein subunit , genetics
Octamer transcription factor 1 ( OCT 1) is an androgen receptor ( AR )‐interacting partner and regulates the expression of target genes in prostate cancer cells. However, the function of OCT 1 in castration‐resistant prostate cancer ( CRPC ) is not fully understood. In the present study, we used 22Rv1 cells as AR ‐positive CRPC model cells to analyze the role of OCT 1 in CRPC . We showed that OCT 1 knockdown suppressed cell proliferation and migration of 22Rv1 cells. Using microarray analysis, we identified four AR and OCT 1‐target genes, disks large‐associated protein 5 ( DLGAP 5), kinesin family member 15 ( KIF 15), non‐ SMC condensin I complex subunit G ( NCAPG ), and NDC 80 kinetochore complex component ( NUF 2) in 22Rv1 cells. We observed that knockdown of DLGAP 5 and NUF 2 suppresses growth and migration of 22Rv1 cells. Furthermore, immunohistochemical analysis showed that positive expression of DLGAP 5 in prostate cancer specimens is related to poor cancer‐specific survival rates of patients. Notably, enhanced expression of DLGAP 5 was observed in CRPC tissues of patients. Thus, our findings suggest that these four genes regulated by the AR / OCT 1 complex could have an important role in CRPC progression.