Open AccessMicroRNA‐132‐3p inhibits tumor malignant progression by regulating lysosomal‐associated protein transmembrane 4 beta in breast cancer
Author(s) -
Li Sha,
Xu JianJun,
Zhang QingYun
Publication year - 2019
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.14164
Subject(s) - carcinogenesis , oncogene , cancer research , breast cancer , microrna , biology , cancer , protein kinase b , pi3k/akt/mtor pathway , transmembrane protein , western blot , tumor progression , untranslated region , messenger rna , signal transduction , gene , microbiology and biotechnology , cell cycle , receptor , genetics
Lysosomal‐associated protein transmembrane 4 beta ( LAPTM 4B), a proto‐oncogene, has been shown to be a positive modulator in cancer progression. However, the mechanism of LAPTM 4B regulation is not fully elucidated. Aberrant micro RNA s (mi RNA s) can regulate gene expression by interfering with target transcripts and/or translation to exert tumor‐suppressive or oncogenic effects in breast cancer. In the present study, miR‐132‐3p, which was predicted by relevant software, was confirmed to directly bind to the 3′ untranslated region (3′ UTR ) of LAPTM 4B and negatively regulate its expression in luciferase reporter and western blot assays. Subsequently, we validated that miR‐132‐3p was downregulated in breast cancer tissues. Receiver‐operating characteristic curve analysis indicated that miR‐132‐3p had accurate diagnostic value, and a Kaplan‐Meier and Cox regression model showed that miR‐132‐3p was a potential prognostic marker for recurrence, showing low levels in breast cancer patients. In addition, we showed that miR‐132‐3p was inversely correlated with LAPTM 4B expression in the above samples. Functionally, miR‐132‐3p suppressed the migration and invasion of breast carcinoma cells through LAPTM 4B by mediating epithelial‐mesenchymal transition signals, and partially reversed the carcinogenic effects of LAPTM 4B by inhibiting the PI 3K‐ AKT ‐ mTOR signaling pathway. Taken together, these findings provide the first comprehensive analysis of miR‐132‐3p as a direct LAPTM 4B‐targeted mi RNA , and shed light on miR‐132‐3p/ LAPTM 4B as a significant functional axis involved in the oncogenesis and metastasis of breast cancer.