Open AccessComprehensive immune characterization and T‐cell receptor repertoire heterogeneity of retroperitoneal liposarcoma
Author(s) -
Yan Liang,
Wang Zhen,
Cui Can,
Guan Xiaoya,
Dong Bin,
Zhao Min,
Wu Jianhui,
Tian Xiuyun,
Hao Chunyi
Publication year - 2019
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.14161
Subject(s) - t cell receptor , immune system , biology , foxp3 , pathology , t cell , immunohistochemistry , cd8 , immunology , cancer research , medicine
Abstract Retroperitoneal liposarcoma ( RLPS ) is one of the most common subtypes of retroperitoneal soft tissue sarcomas and lacks effective treatment. This study aimed to provide a thorough profile of immune characteristics of RLPS . This study included 56 RLPS patients. Multisite tumor tissues were collected from 16 patients. Immunohistochemistry was carried out to identify CD 4 + , CD 8 + , FoxP3 + , CD 20 + , or programmed cell death‐1 ( PD ‐1) + tumor infiltrating lymphocytes ( TIL s) and Programmed cell death ligand‐1 ( PD ‐L1) expression in tumor tissues. Ultradeep sequencing of T‐cell receptor ( TCR ) β‐chain gene was carried out in 42 tumor samples as well as peripheral blood samples collected from 6 patients. In RLPS , TIL s were distributed in 3 patterns and T cells were more prevalent than B cells. Generally, the proportion of TIL s decreased and PD ‐L1 expression increased with tumor progression. Patients with higher PD ‐1/ PD ‐L1 expression tended to have poorer prognosis, whereas patients with tertiary lymphoid structure tended to have a favorable disease‐free survival. Although T‐cell clones in tumors were quite different from those in peripheral blood, TCR sequencing showed low TCR repertoire reads as well as polyclonal status within tumors, which indicated limited T cell response in the tumors. Both TIL s distribution and TCR repertoires suggested spatial immune heterogeneity in RLPS . Our research described the immune landscape of RLPS , and suggested RLPS might be a kind of tumor with low T cell infiltration as well as great immune heterogeneity. Therefore, strategies that can facilitate lymphocytic infiltration and immune reactivity need to be developed in the future to improve the efficacy of immunotherapy.