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Long non‐coding  RNA MIF ‐ AS 1 (lnc MIF ‐ AS 1) has been found to be upregulated in the tumor tissues of gastric cancer; however, its importance for the progression of gastric cancer remains unknown. Thus, the present study was designed to determine the role of the lnc MIF ‐ AS 1‐based signal transduction pathway in mediating the proliferation and apoptosis of gastric cancer cells. Differentially expressed lnc RNA s and  mRNA s were screened out using microarray analysis, based on the published data ( GSE 63288), and validated using quantitative  RT ‐ PCR . Target relationships between lnc RNA ‐micro RNA (mi RNA ) and mi RNA ‐ mRNA  were predicted by bioinformatics analysis and verified by dual‐luciferase reporter assay. Protein expression of  NDUFA 4,  COX 6C and  COX 5B was detected by western blot. Cell proliferation, cell cycle and apoptosis were determined using colony formation assay and flow cytometry analysis. Oxidative phosphorylation in gastric cancer cells was assessed by levels of oxygen consumption and  ATP  synthase activity. Expression of lnc MIF ‐ AS 1 and  NDUFA 4 were upregulated in gastric cancer tissues and cells as compared with non‐cancerous gastric tissues and cells ( P  <   .05). MiR‐212‐5p was identified as the most important mi RNA  linker between lnc MIF ‐ AS 1 and  NDUFA 4, which was negatively regulated by lnc MIF ‐ AS 1 and its depletion is the main cause of  NDUFA 4 overexpression ( P  <   .01). The upregulated expression of  NDUFA 4 then greatly promoted the proliferation and decreased the apoptosis of gastric cancer cells through activation of the oxidative phosphorylation pathway. Taken together, the present study implies that inhibition of lnc MIF ‐ AS 1/miR‐212‐5p/ NDUFA 4 signal transduction may provide a promising therapeutic target for the treatment of gastric cancer.

Long non‐coding RNA MIF ‐ AS 1 promotes gastric cancer cell proliferation and reduces apoptosis to upregulate NDUFA 4