Open AccessAlternating magnetic field enhances cytotoxicity of Compound C
Author(s) -
Akimoto Taisuke,
Umemura Masanari,
Nagasako Akane,
Ohtake Makoto,
Fujita Takayuki,
Yokoyama Utako,
Eguchi Haruki,
Yamamoto Tetsuya,
Ishikawa Yoshihiro
Publication year - 2018
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.13781
Subject(s) - cytotoxicity , apoptosis , ampk , reactive oxygen species , p38 mitogen activated protein kinases , chemistry , cell culture , mapk/erk pathway , in vitro , cancer cell , autophagy , cancer research , kinase , pharmacology , protein kinase a , cancer , biochemistry , biology , medicine , genetics
Abstract We previously reported the efficacy of anti‐cancer therapy with hyperthermia using an alternating magnetic field ( AMF ) and a magnetic compound. In the course of the study, unexpectedly, we found that an AMF enhances the cytotoxicity of Compound C, an activated protein kinase ( AMPK ) inhibitor, although this compound is not magnetic. Therefore, we examined the cellular mechanism of AMF ‐induced cytotoxicity of Compound C in cultured human glioblastoma ( GB ) cells. An AMF (280 kH z, 250 Arms) for 30 minutes significantly enhanced the cytotoxicity of Compound C and promoted apoptosis towards several human GB cell lines in vitro. The AMF also increased Compound C‐induced cell‐cycle arrest of GB cells at the G2 phase and, thus, inhibited cell proliferation. The AMF increased Compound C‐induced reactive oxygen species production. Furthermore, the AMF decreased ERK phosphorylation in the presence of Compound C and suppressed the protective autophagy induced by this compound. The application of an AMF in cancer chemotherapy may be a simple and promising method, which might reduce the doses of drugs used in future cancer treatment and, therefore, the associated side effects.