English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

EWS ‐ FLI 1 constitutes an oncogenic transcription factor that plays key roles in Ewing sarcoma development and maintenance. We have recently succeeded in generating an ex vivo mouse model for Ewing sarcoma by introducing  EWS ‐ FLI 1 into embryonic osteochondrogenic progenitors. The model well recapitulates the biological characteristics, small round cell morphology, and gene expression profiles of human Ewing sarcoma. Here, we clarified the global  DNA  binding properties of  EWS ‐ FLI 1 in mouse Ewing sarcoma.  GGAA  microsatellites were found to serve as binding sites of  EWS ‐ FLI 1 albeit with less frequency than that in human Ewing sarcoma; moreover, genomic distribution was not conserved between human and mouse. Nevertheless,  EWS ‐ FLI 1 binding sites within  GGAA  microsatellites were frequently associated with the histone H3K27Ac enhancer mark, suggesting that  EWS ‐ FLI 1 could affect global gene expression by binding its target sites. In particular, the Fox transcription factor binding motif was frequently observed within  EWS ‐ FLI 1 peaks and Foxq1 was identified as the cooperative partner that interacts with the  EWS  portion of  EWS ‐ FLI 1.  Trib1  and  Nrg1  were demonstrated as target genes that are co‐regulated by  EWS ‐ FLI 1 and Foxq1, and are important for cell proliferation and survival of Ewing sarcoma. Collectively, our findings present novel aspects of  EWS ‐ FLI 1 function as well as the importance of  GGAA  microsatellites.

EWS ‐ FLI 1 regulates a transcriptional program in cooperation with Foxq1 in mouse Ewing sarcoma