Open AccessRibosome display and selection of single‐chain variable fragments effectively inhibit growth and progression of microspheres in vitro and in vivo
Author(s) -
Huang Shangke,
Feng Lu,
An Gaili,
Zhang Xiaojin,
Zhao Zixuan,
Han Rui,
Lei Fuxi,
Zhang Yujiao,
Luo Anqi,
Jing Xin,
Zhao Lin,
Gu Shanzhi,
Zhao Xinhan,
Zhang Lingxiao
Publication year - 2018
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.13574
Subject(s) - in vivo , in vitro , microbiology and biotechnology , cancer research , chemistry , biology , biochemistry , genetics
Distinguishing the surface markers of cancer stem cells ( CSC s) is a useful method for early diagnosis and treatment of tumors, as CSC s may participate in tumorigenesis and metastasis by migrating into the circulatory system. However, the potential targets of CSC s are expressed at low levels in the natural state and are always changing. Thus, dynamic screening has been reported to be an effective measure for exploring CSC markers. In recent years, diverse single‐chain variable fragments (scFvs) have been widely used in immunotherapy. In this study, we determined that the scFvs, screened using RD , had a high affinity to microspheres and could inhibit their progression. We also observed that the selected scFvs underwent evolution in vitro, and antitumor‐associated proteins were successfully expressed. Combined with chemotherapy, the scFvs had a synergistic effect on the inhibition of the microspheres’ progression in vitro and in vivo, which could be ascribed to their high affinity for stem‐like cells and the inhibition of the microspheres’ collective behaviors. In addition, proteins inhibiting CD 44 + / CD 24 + and MAPK were involved. Our data indicated that dynamic screening of the scFvs in a natural state was of great significance in the inhibition of the microspheres in vitro and in vivo.