Open AccessLong non‐coding RNA RP 11‐552M11.4 promotes cells proliferation, migration and invasion by targeting BRCA 2 in ovarian cancer
Author(s) -
Huang Kejin,
Geng Jiashi,
Wang Jing
Publication year - 2018
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.13552
Subject(s) - rna , biology , cell growth , matrigel , microbiology and biotechnology , cancer research , ovarian cancer , long non coding rna , propidium iodide , apoptosis , annexin a2 , cell , transfection , annexin , cell culture , cancer , gene , flow cytometry , programmed cell death , angiogenesis , genetics
The present study aimed to investigate the effect of long non‐coding RNA (lnc RNA ) RP 11‐552M11.4 on cell proliferation, apoptosis, migration and invasion as well as its targeting genes in epithelial ovarian cancer ( EOC ) cells. Lnc RNA RP 11‐552M11.4 expression was detected in 67 tumor tissues and paired adjacent tissues obtained from EOC patients. lnc RNA RP 11‐552M11.4 mimic/inhibitor plasmids were transferred into ovarian cancer cells ( SKOV 3, A‐2780) and normal ovarian epithelial cells ( IOSE 80 cells). In addition, rescue experiment was carried out by transferring BRCA 2 inhibitor&lnc RNA RP 11‐552M11.4 inhibitor plasmids into SKOV 3 and A‐2780 cells. qPCR , western blot, CKK ‐8, Annexin V/propidium iodide ( AV / PI ), wound‐healing and Matrigel invasion assays were carried out to detect RNA expression, protein expression, cell proliferation, apoptosis, migration, and invasion, respectively. Lnc RNA RP 11‐552M11.4 expression was elevated in tumor tissues compared with paired adjacent tissues and correlated with higher pathological grade, International Federation of Gynecology and Obstetrics stage and worse overall survival in EOC patients. Lnc RNA RP 11‐552M11.4 promoted SKOV 3 cell proliferation, migration and invasion whereas it inhibited apoptosis. Rescue experiment and luciferase reporter assay showed that lnc RNA RP 11‐552M11.4 regulated SKOV 3 cells functions through binding BRCA 2. Further experiments in A‐2780 cells also validated that lnc RNA RP 11‐552M11.4 induced A‐2780 cell proliferation while repressing apoptosis by targeting BRCA 2. In addition, upregulation of lnc RNA RP 11‐552M11.4 increased IOSE 80 cell proliferation, migration and invasion while decreasing apoptosis. In conclusion, lnc RNA RP 11‐552M11.4 correlates with worse prognosis, and promotes cell proliferation, migration, invasion, and inhibits cell apoptosis by down‐regulating BRCA 2 in EOC .