Open AccessX‐ray‐enhanced cancer cell migration requires the linker of nucleoskeleton and cytoskeleton complex
Author(s) -
Imaizumi Hiromasa,
Sato Katsutoshi,
Nishihara Asuka,
Minami Kazumasa,
Koizumi Masahiko,
Matsuura Nariaki,
Hieda Miki
Publication year - 2018
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.13545
Subject(s) - cell migration , microbiology and biotechnology , chromatin , lamin , cytoskeleton , biology , dna damage , cell , chemistry , nucleus , dna , genetics
The linker of nucleoskeleton and cytoskeleton ( LINC ) complex is a multifunctional protein complex that is involved in various processes at the nuclear envelope, including nuclear migration, mechanotransduction, chromatin tethering and DNA damage response. We recently showed that a nuclear envelope protein, Sad1 and UNC 84 domain protein 1 ( SUN 1), a component of the LINC complex, has a critical function in cell migration. Although ionizing radiation activates cell migration and invasion in vivo and in vitro, the underlying molecular mechanism remains unknown. Here, we examined the involvement of the LINC complex in radiation‐enhanced cell migration and invasion. A sublethal dose of X‐ray radiation promoted human breast cancer MDA ‐ MB ‐231 cell migration and invasion, whereas carbon ion beam radiation suppressed these processes in a dose‐dependent manner. Depletion of SUN 1 and SUN 2 significantly suppressed X‐ray‐enhanced cell migration and invasion. Moreover, depletion or overexpression of each SUN 1 splicing variant revealed that SUN 1_888 containing 888 amino acids of SUN 1 but not SUN 1_916 was required for X‐ray‐enhanced migration and invasion. In addition, the results suggested that X‐ray irradiation affected the expression level of SUN 1 splicing variants and a SUN protein binding partner, nesprins. Taken together, our findings supported that the LINC complex contributed to photon‐enhanced cell migration and invasion.