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Elevated expression of JAM ‐A promotes neoplastic properties of lung adenocarcinoma
Author(s) -
Magara Kazufumi,
Takasawa Akira,
Osanai Makoto,
Ota Misaki,
Tagami Yohei,
Ono Yusuke,
Takasawa Kumi,
Murata Masaki,
Hirohashi Yoshihiko,
Miyajima Masahiro,
Yamada Gen,
Hasegawa Tadashi,
Sawada Norimasa
Publication year - 2017
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.13385
Subject(s) - atypical adenomatous hyperplasia , adenocarcinoma , carcinogenesis , cancer research , apoptosis , biology , hyperplasia , immunohistochemistry , in vivo , lung cancer , cell growth , cell adhesion molecule , cell , cancer , pathology , immunology , medicine , endocrinology , genetics
A cell–cell adhesion protein, junctional adhesion molecule‐A ( JAM ‐A), has been shown to be involved in neoplasia of various organs. However, the fundamental role of JAM ‐A in tumorigenesis is still under debate because dysregulated expression of this protein has distinct effects, playing opposite roles in carcinogenesis depending on the target tissues. In the present study, we found elevated levels of JAM ‐A expression in lung adenocarcinoma and its preinvasive lesions, including atypical adenomatous hyperplasia and adenocarcinoma in situ by immunohistochemistry. We also showed that suppression of constitutive JAM ‐A expression conferred target cells with increased susceptibility to apoptosis in lung adenocarcinoma cells. Consequently, inhibition of JAM ‐A activity decreased colony‐forming capability in vitro and tumorigenicity in vivo . The transformed phenotype following suppression of JAM ‐A expression was sufficient to reduce motile and invasive capacities. Importantly, knockout of JAM ‐A had striking effects on cells. Our observations suggest that increased expression of JAM ‐A promotes neoplasia of lung adenocarcinoma. In addition, an anti‐ JAM ‐A antibody efficiently reduced cell proliferation and provoked apoptosis, indicating the potential feasibility of JAM ‐A‐inhibitory cancer therapy.