Open AccessLong non‐coding RNA TUG 1 promotes migration and invasion by acting as a ce RNA of miR‐335‐5p in osteosarcoma cells
Author(s) -
Wang Yong,
Yang Tao,
Zhang Zhen,
Lu Ming,
Zhao Wei,
Zeng Xiandong,
Zhang Weiguo
Publication year - 2017
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.13201
Subject(s) - osteosarcoma , rna , oncogene , cancer research , competing endogenous rna , messenger rna , chemistry , biology , long non coding rna , cell , gene , biochemistry , cell cycle
Long non‐coding RNA (lnc RNA ) have been the focus of increasing attention due to the role they play in many diseases, including osteosarcoma. The function of taurine upregulated gene 1 ( TUG 1) and its mechanism in osteosarcoma remain unclear. In our research, we found that TUG 1 was elevated and correlated with a poor prognosis in osteosarcoma patients. In addition, the following functional experiment showed that decreased TUG 1 could remarkably inhibit osteosarcoma cell migration and invasion, indicating that TUG 1 functioned as an oncogene in osteosarcoma. Moreover, we revealed that TUG 1 and Rho‐associated coiled‐coil‐containing protein kinase 1 ( ROCK 1), a metastasis‐related gene targeted by micro RNA ‐335‐5p (miR‐335‐5p), had the same miR‐335‐5p combining site. The subsequent luciferase assay verified TUG 1 was a target of miR‐335‐5p. Furthermore, the results of a real‐time quantitative PCR showed that TUG 1 and miR‐335‐5p could affect each other's expression. respectively. Finally, we affirmed that TUG 1 affected ROCK 1 expression and ROCK 1‐mediated migration/invasion by working as a competitive endogenous RNA (ce RNA ) via miR‐335‐5p. In summary, the findings of this study, based on ce RNA theory, combining the research foundation of miR‐335‐5p and ROCK 1, and taking TUG 1 as a new study point, provide new insight into molecular‐level reversing migration and invasion of osteosarcoma.