Open AccessSialyltransferase ST 3 GAL 6 mediates the effect of micro RNA ‐26a on cell growth, migration, and invasion in hepatocellular carcinoma through the protein kinase B/mammalian target of rapamycin pathway
Author(s) -
Sun Mingming,
Zhao Xuzi,
Liang Leilei,
Pan Xufeng,
Lv Hao,
Zhao Yongfu
Publication year - 2017
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.13128
Subject(s) - pi3k/akt/mtor pathway , protein kinase b , cell growth , downregulation and upregulation , cancer research , cell , biology , western blot , cell migration , signal transduction , microbiology and biotechnology , chemistry , biochemistry , gene
Aberrant sialylation profiles on the cell surface have been recognized for their potential diagnostic value in identifying the regulation of tumor properties in several cancers, including hepatocellular carcinoma ( HCC ). Recently, increasing evidence has suggested that the deregulation of micro RNA (mi RNA ) is a common feature in human cancers. In this study, we found obvious upregulation of sialyltransferase ST 3 GAL 6 both in HCC cell lines and in tissue samples. The altered expression of ST 3 GAL 6 was found to correlate with cell proliferation, migration, and invasion ability in HCC . Further investigation showed that miR‐26a negatively regulated ST 3 GAL 6, inducing the suppression of cell proliferation, migration, and invasion in vitro . Moreover, we identified the protein kinase B/mammalian target of rapamycin (Akt/ mTOR ) pathway as the target of ST 3 GAL 6 based on Western blot analysis. Analysis of a xenograft mouse model showed that miR‐26a significantly reduced tumor growth by suppressing activation of the Akt/ mTOR pathway by directly targeting ST 3 GAL 6. In conclusion, these data indicate that ST 3 GAL 6 promotes cell growth, migration, and invasion and mediates the effect of miR‐26a through the Akt/ mTOR signaling pathway in HCC.