Open AccessZFP 36L2 promotes cancer cell aggressiveness and is regulated by antitumor micro RNA ‐375 in pancreatic ductal adenocarcinoma
Author(s) -
Yonemori Keiichi,
Seki Naohiko,
Kurahara Hiroshi,
Osako Yusaku,
Idichi Tetsuya,
Arai Takayuki,
Koshizuka Keiichi,
Kita Yoshiaki,
Maemura Kosei,
Natsugoe Shoji
Publication year - 2017
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.13119
Subject(s) - cancer research , microrna , gene silencing , pancreatic cancer , cancer , zinc finger , ectopic expression , cell culture , medicine , biology , gene , transcription factor , genetics
Due to its aggressive nature, pancreatic ductal adenocarcinoma ( PDAC ) is one of the most lethal and hard‐to‐treat malignancies. Recently developed targeted molecular strategies have contributed to remarkable improvements in the treatment of several cancers. However, such therapies have not been applied to PDAC . Therefore, new treatment options are needed for PDAC based on current genomic approaches. Expression of micro RNA ‐375 ( miR‐375 ) was significantly reduced in mi RNA expression signatures of several types of cancers, including PDAC . The aim of the present study was to investigate the functional roles of miR‐375 in PDAC cells and to identify miR‐375 ‐regulated molecular networks involved in PDAC aggressiveness. The expression levels of miR‐375 were markedly downregulated in PDAC clinical specimens and cell lines ( PANC ‐1 and SW 1990). Ectopic expression of miR‐375 significantly suppressed cancer cell proliferation, migration and invasion. Our in silico and gene expression analyses and luciferase reporter assay showed that zinc finger protein 36 ring finger protein‐like 2 ( ZFP 36L2 ) was a direct target of miR‐375 in PDAC cells. Silencing ZFP 36L2 inhibited cancer cell aggressiveness in PDAC cell lines, and overexpression of ZFP 36L2 was confirmed in PDAC clinical specimens. Interestingly, Kaplan–Meier survival curves showed that high expression of ZFP 36L2 predicted shorter survival in patients with PDAC . Moreover, we investigated the downstream molecular networks of the miR‐375/ ZFP 36L2 axis in PDAC cells. Elucidation of tumor‐suppressive miR‐375 ‐mediated PDAC molecular networks may provide new insights into the potential mechanisms of PDAC pathogenesis.