Open AccessEstablishment of a novel method to evaluate peritoneal microdissemination and therapeutic effect using luciferase assay
Author(s) -
Takahashi Ryo,
Yokobori Takehiko,
Osone Katsuya,
Tatsuki Hironori,
Takada Takahiro,
Suto Toshinaga,
Yajima Reina,
Kato Toshihide,
Fujii Takaaki,
Tsutsumi Souichi,
Kuwano Hiroyuki,
Asao Takayuki
Publication year - 2016
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.12872
Subject(s) - micrometastasis , luciferase , in vivo , peritoneal cavity , medicine , ex vivo , cancer research , pharmacology , pathology , cancer , biology , cell culture , surgery , metastasis , transfection , genetics , microbiology and biotechnology
Peritoneal dissemination is a major cause of recurrence in patients with malignant tumors in the peritoneal cavity. Effective anticancer agents and treatment protocols are necessary to improve outcomes in these patients. However, previous studies using mouse models of peritoneal dissemination have not detected any drug effect against peritoneal micrometastasis. Here we used the luciferase assay to evaluate peritoneal micrometastasis in living animals and established an accurate mouse model of early peritoneal microdissemination to evaluate tumorigenesis and drug efficacy. There was a positive correlation between luminescence intensity in in vivo luciferase assay and the extent of tumor dissemination evaluated by ex vivo luciferase assay and mesenteric weight. This model has advantages over previous models because optimal luciferin concentration without cell damage was validated and peritoneal microdissemination could be quantitatively evaluated. Therefore, it is a useful model to validate peritoneal micrometastasis formation and to evaluate drug efficacy without killing mice.