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The precise control of micro RNA  (mi RNA ) biosynthesis is crucial for gene regulation. Lin28A and Lin28B are selective inhibitors of biogenesis of let‐7 miRNAs involved in development and tumorigenesis. Lin28A selectively inhibits let‐7 biogenesis through cytoplasmic uridylation of precursor let‐7 by  TUT 4 terminal uridyl transferase and subsequent degradation by Dis3l2 exonuclease. However, a role of this uridylation pathway remains unclear in let‐7 blockade by Lin28B, a paralog of Lin28A, while Lin28B is reported to engage a distinct mechanism in the nucleus to suppress let‐7. Here we revisit a functional link between Lin28B and the uridylation pathway with a focus on let‐7 metabolism in cancer cells. Both Lin28A and Lin28B interacted with Dis3l2 in the cytoplasm, and silencing of Dis3l2 upregulated uridylated pre‐let‐7 in both Lin28A‐ and Lin28B‐expressing cancer cell lines. In addition, we found that amounts of let‐7 precursors influenced intracellular localization of Lin28B. Furthermore, we found that  MCPIP 1 (Zc3h12a) ribonuclease was also involved in degradation of both non‐uridylated and uridylated pre‐let‐7. Cancer transcriptome analysis showed association of expression levels of Lin28B and uridylation pathway components,  TUT 4 and Dis3l2, in various human cancer cells and hepatocellular carcinoma. Collectively, these results suggest that cytoplasmic uridylation pathway actively participates in blockade of let‐7 biogenesis by Lin28B.

A role of uridylation pathway for blockade of let‐7 micro RNA biogenesis by Lin28B