English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

Three‐dimensional (3D) cell culture is beneficial for physiological studies of tumor cells, due to its potential to deliver a high quantity of cell culture information that is representative of the cancer microenvironment and predictive of drug responses  in vivo . Currently, gel‐associated or matrix‐associated 3D cell culture is comprised of intricate procedures that often result in experimental complexity. Therefore, we developed an innovative anti‐cancer drug sensitivity screening technique for 3D cell culture on NanoCulture Plates ( NCP ) by employing the imaging device BioStation  CT . Here, we showed that the human breast cancer cell lines  BT 474 and T47D form multicellular spheroids on  NCP  plates and compared their sensitivity to the anti‐cancer drugs trastuzumab and paclitaxel using the BioStation  CT . The anticancer drugs reduced spheroid migration velocity and suppressed spheroid fusion. In addition, primary cells derived from the human breast cancer tissues B58 and B61 grown on  NCP  plates also exhibited similar drug sensitivity. These results were in good agreement with the conventional assay method using  ATP  quantification. We confirmed the antitumor effects of the drugs on cells seeded in 96‐well plates using the BioStation  CT  imaging technique. We expect this method to be useful in research for new antitumor agents and for drug sensitivity tests in individually‐tailored cancer treatments.

Time‐lapse imaging assay using the BioStation CT : A sensitive drug‐screening method for three‐dimensional cell culture