Open AccessmiR‐185‐3p regulates nasopharyngeal carcinoma radioresistance by targeting WNT 2B in vitro
Author(s) -
Li Guo,
Wang Yunyun,
Liu Yong,
Su Zhongwu,
Liu Chao,
Ren Shuling,
Deng Tengbo,
Huang Donghai,
Tian Yongquan,
Qiu Yuanzheng
Publication year - 2014
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.12555
Subject(s) - radioresistance , nasopharyngeal carcinoma , in vitro , wnt signaling pathway , cancer research , biology , microbiology and biotechnology , medicine , cell culture , radiation therapy , signal transduction , genetics
Aberrant micro RNA (mi RNA ) expression contributes to a series of malignant cancer behaviors, including radioresistance. Our previous study showed differential expression of miR‐185‐3p in post‐radiation nasopharyngeal carcinoma ( NPC ) cells. To investigate the role of miR‐185‐3p in NPC radioresistance, CNE ‐2 and 5‐8F cells were transfected with miR‐185‐3p mimic and miR‐185‐3p inhibitor, respectively. CCK ‐8 assay and colony formation experiment confirmed that the expression of miR‐185‐3p affected the radioresistance of NPC cells. A negative correlation between miR‐185‐3p and WNT 2B expression was observed in NPC cells and tissues. Luciferase reporter assays confirmed that miR‐185‐3p directly targeted the coding region of WNT 2B. Furthermore, we found radioresistance decreased in WNT 2B‐silenced NPC cells. Activation of the WNT 2B/β‐catenin pathway was accompanied by epithelial–mesenchymal transition biomarker changes in NPC . We concluded that miR‐185‐3p contributed to the radioresistance of NPC via modulation of WNT 2B expression in vitro .