Open AccessRelation between outcomes and expression of estrogen receptor‐α phosphorylated at Ser 167 in endometrioid endometrial cancer
Author(s) -
Kato Eiichi,
Orisaka Makoto,
Kurokawa Tetsuji,
Chino Yoko,
Fujita Yuko,
Shinagawa Akiko,
Yoshida Yoshio
Publication year - 2014
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.12491
Subject(s) - p70 s6 kinase 1 , estrogen receptor , endometrial cancer , immunohistochemistry , phosphorylation , mapk/erk pathway , ribosomal protein s6 , cancer research , estrogen , biology , pi3k/akt/mtor pathway , kinase , medicine , endocrinology , cancer , signal transduction , microbiology and biotechnology , breast cancer
Both ligand‐dependent and ligand‐independent activation of estrogen receptor (ER)α is modulated by receptor phosphorylation and results in activation of the ERα‐dependent pathways that are involved in endometrioid endometrial cancer ( EEC ) pathogenesis. It is also known that the mammalian target of rapamycin ( mTOR )/p70 S6 kinase 1 ( S6K 1) and MAPK /p90 ribosomal S6 kinase ( RSK ) signaling pathways coordinately regulate phosphorylated‐ ER α at Ser 167 (p‐Ser 167 ‐ ER α). However, the expression of p‐Ser 167 ‐ ER α in EEC and its prognostic role in ECC is largely unexplored. The purpose of the present study was to investigate the expression of p‐Ser 167 ‐ ER α in ECC and its relationship with prognosis. Immunohistochemical staining of primary EEC surgical specimens ( n = 103) was carried out using antibodies specific for p‐Ser 167 ‐ ER α and for p‐ mTOR /p‐S6K1 and p‐ MAPK /p‐ RSK . The correlation of p‐Ser 167 ‐ ER α expression with clinicopathological features and survival of ECC was studied. Patients that were positive for nuclear p‐Ser 167 ‐ ER α had significantly shorter relapse‐free survival, and although the result was not significant, levels of nuclear p‐Ser 167 ‐ERα tended to be higher in advanced‐stage ECC patients. Nuclear p‐Ser 167 ‐ ER α was significantly positively correlated with p‐ MAPK and p‐ S6K 1, and with significantly shorter relapse‐free survival in EEC .