Open AccessPeripheral blood mitochondrial DNA content, A10398G polymorphism, and risk of breast cancer in a Han Chinese population
Author(s) -
Jiang Huangang,
Zhao Hong,
Xu Hui,
Hu Liu,
Wang Wenbo,
Wei Yuehua,
Wang You,
Peng Xiaohong,
Zhou Fuxiang
Publication year - 2014
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.12412
Subject(s) - breast cancer , odds ratio , mitochondrial dna , confidence interval , medicine , oncology , cancer , peripheral blood , biology , gastroenterology , immunology , genetics , gene
It has been reported that quantitative alterations and sequence variations of mt DNA are associated with the onset and progression of particular types of tumor. However, the relationship between mt DNA content, certain mt DNA polymorphisms in peripheral blood leukocytes and breast cancer risk remain obscure. This study was undertaken to investigate whether mt DNA content and the A10398G polymorphism in peripheral blood leukocytes could be used as risk predictors for breast cancer in Han Chinese women. Blood samples were obtained from a total of 506 breast cancer patients and 520 matched healthy controls. The mt DNA content was measured by using quantitative real‐time PCR assay; A10398G polymorphism was determined by PCR ‐ RFLP assay. There was no statistically significant difference between cases and controls in terms of peripheral blood mt DNA content or A10398G polymorphism. However, further analysis suggested that the risk of breast cancer was associated with decreased mt DNA content in premenopausal women ( P = 0.001; odds ratio = 0.54; 95% confidence interval, 0.38–0.77), with increased mt DNA content in postmenopausal women ( P = 0.027; odds ratio = 1.49; 95% confidence interval, 1.05–2.11). In addition, the associations between mt DNA content and several clinicopathological parameters of cases such as age, menopausal status, and number of pregnancies and live births were observed. This case–control study indicated that the peripheral blood mt DNA content might be a potential biomarker to evaluate the risk of breast cancer for selected Chinese women.