English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

Summary  Human induced pluripotent stem cells (hi PSC s), like embryonic stem cells, are under intense investigation for novel approaches to model disease and for regenerative therapies. Here, we describe the derivation and characterization of hi PSC s from a variety of sources and show that, irrespective of origin or method of reprogramming, hi PSC s can be differentiated on  OP 9 stroma towards a multi‐lineage haemo‐endothelial progenitor that can contribute to  CD 144 +  endothelium,  CD 235a +  erythrocytes (myeloid lineage) and  CD 19 +  B lymphocytes (lymphoid lineage). Within the erythroblast lineage, we were able to demonstrate by single cell analysis (flow cytometry), that hi PSC ‐derived erythroblasts express alpha globin as previously described, and that a sub‐population of these erythroblasts also express haemoglobin F (HbF), indicative of fetal definitive erythropoiesis. More notably however, we were able to demonstrate that a small sub‐fraction of HbF positive erythroblasts co‐expressed HbA in a highly heterogeneous manner, but analogous to cord blood‐derived erythroblasts when cultured using similar methods. Moreover, the HbA expressing erythroblast population could be greatly enhanced (44·0 ± 6·04%) when a defined serum‐free approach was employed to isolate a  CD 31 +   CD 45 +  erythro‐myeloid progenitor. These findings demonstrate that hi PSC s may represent a useful alternative to standard sources of erythrocytes ( RBC s) for future applications in transfusion medicine.

Human induced pluripotent stem cell derived erythroblasts can undergo definitive erythropoiesis and co‐express gamma and beta globins