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Optimization of a drug transporter probe cocktail: potential screening tool for transporter‐mediated drug–drug interactions
Author(s) -
Stopfer Peter,
Giessmann Thomas,
Hohl Kathrin,
Hutzel Sabine,
Schmidt Sven,
Gansser Dietmar,
Ishiguro Naoki,
Taub Mitchell E.,
Sharma Ashish,
Ebner Thomas,
Müller Fabian
Publication year - 2018
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1111/bcp.13609
Subject(s) - rosuvastatin , pharmacokinetics , furosemide , digoxin , pharmacology , crossover study , metformin , drug interaction , quinidine , rosuvastatin calcium , drug , medicine , area under the curve , bioequivalence , chemistry , heart failure , placebo , alternative medicine , pathology , insulin
Aims Previous pharmacokinetic characterization of a transporter probe cocktail containing digoxin (P‐gp), furosemide (OAT1, OAT3), metformin (OCT2, MATE1, MATE2‐K) and rosuvastatin (OATP1B1, OATP1B3, BCRP) in healthy subjects showed increases in rosuvastatin systemic exposure compared to rosuvastatin alone. In this trial, the doses of metformin and furosemide as putative perpetrators were reduced to eliminate their drug–drug interaction (DDI) with rosuvastatin. Methods In a randomized, open‐label, single‐centre, five‐treatment, five‐period crossover trial, 30 healthy male subjects received as reference treatments separately 0.25 mg digoxin, 1 mg furosemide, 10 mg metformin and 10 mg rosuvastatin, and as test treatment all four drugs administered together as a cocktail. Primary pharmacokinetic endpoints were AUC 0‐tz (area under the plasma concentration–time curve from time zero to the last quantifiable concentration) and C max (maximum plasma concentration) of each probe drug. Results Geometric mean ratios and 90% confidence intervals of test (cocktail) to reference (single drug) for AUC 0‐tz were 96.4% (88.2–105.3%) for digoxin, 102.6% (93.8–112.3%) for furosemide, 97.5% (93.5–101.6%) for metformin and 105.0% (96.4–114.4%) for rosuvastatin, indicating lack of interaction. The same analysis for C max and for pharmacokinetic parameters of urinary excretion of all cocktail components also indicated no DDI. Conclusions Digoxin (0.25 mg), furosemide (1 mg), metformin (10 mg) and rosuvastatin (10 mg) exhibit no mutual pharmacokinetic interactions and are well tolerated administered as a cocktail. The cocktail is thus optimized and has the potential to be used as a screening tool for clinical investigation of transporter‐mediated DDI.