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Phenotypic and functional analysis of bovine peripheral blood dendritic cells before parturition by a novel purification method
Author(s) -
Zhuang Tao,
Urakawa Megumi,
Sato Hidetoshi,
Sato Yuko,
Taguchi Teruaki,
Umino Tsuyoshi,
Katto Shiro,
Tanaka Koutaro,
Yoshimura Kozue,
Takada Naokazu,
Kobayashi Hiroko,
Ito Megumi,
Rose Michael T.,
Kiku Yoshio,
Nagasawa Yuya,
Kitazawa Haruki,
Watanabe Kouichi,
Nochi Tomonori,
Hayashi Tomohito,
Aso Hisashi
Publication year - 2018
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.13014
Subject(s) - peripheral blood mononuclear cell , major histocompatibility complex , immune system , cytokine , immunology , dendritic cell , biology , mhc class ii , antigen , microbiology and biotechnology , t cell , antigen presenting cell , antigen presentation , in vitro , biochemistry
Dendritic cells ( DC s) are specialized antigen presenting cells specializing in antigen uptake and processing, and play an important role in the innate and adaptive immune response. A subset of bovine peripheral blood DC s was identified as CD 172a + / CD 11c + / MHC (major histocompatibility complex) class II + cells. Although DC s are identified at 0.1%–0.7% of peripheral blood mononuclear cells (PBMC ), the phenotype and function of DC s remain poorly understood with regard to maintaining tolerance during the pregnancy. All cattle used in this study were 1 month before parturition. We have established a novel method for the purification of DC s from PBMC using magnetic‐activated cell sorting, and purified the CD 172a + / CD 11c + DC s, with high expression of MHC class II and CD 40, at 84.8% purity. There were individual differences in the expressions of CD 205 and co‐stimulatory molecules CD 80 and CD 86 on DC s. There were positive correlations between expression of cytokine and co‐stimulatory molecules in DC s, and the DC s maintained their immune tolerance, evidenced by their low expressions of the co‐stimulatory molecules and cytokine production. These results suggest that before parturition a half of DC s may be immature and tend to maintain tolerance based on the low cytokine production, and the other DC s with high co‐stimulatory molecules may already have the ability of modulating the T‐cell linage.