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Dendritic cells ( DC s) are specialized antigen presenting cells specializing in antigen uptake and processing, and play an important role in the innate and adaptive immune response. A subset of bovine peripheral blood  DC s was identified as  CD 172a + / CD 11c + / MHC  (major histocompatibility complex) class  II  +  cells. Although  DC s are identified at 0.1%–0.7% of peripheral blood mononuclear cells  (PBMC ), the phenotype and function of  DC s remain poorly understood with regard to maintaining tolerance during the pregnancy. All cattle used in this study were 1 month before parturition. We have established a novel method for the purification of  DC s from  PBMC  using magnetic‐activated cell sorting, and purified the  CD 172a + / CD 11c +   DC s, with high expression of  MHC  class  II  and  CD 40, at 84.8% purity. There were individual differences in the expressions of  CD 205 and co‐stimulatory molecules  CD 80 and  CD 86 on  DC s. There were positive correlations between expression of cytokine and co‐stimulatory molecules in  DC s, and the  DC s maintained their immune tolerance, evidenced by their low expressions of the co‐stimulatory molecules and cytokine production. These results suggest that before parturition a half of  DC s may be immature and tend to maintain tolerance based on the low cytokine production, and the other  DC s with high co‐stimulatory molecules may already have the ability of modulating the T‐cell linage.

Phenotypic and functional analysis of bovine peripheral blood dendritic cells before parturition by a novel purification method