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Establishment and characterization of immortalized bovine endometrial epithelial cells
Author(s) -
Bai Hanako,
Sakurai Toshihiro,
Bai Rulan,
Yamakoshi Sachiko,
Aoki Etsunari,
Kuse Mariko,
Okuda Kiyoshi,
Imakawa Kazuhiko
Publication year - 2014
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12202
Subject(s) - telomerase reverse transcriptase , telomerase , immortalised cell line , transfection , biology , cell culture , fetal bovine serum , microbiology and biotechnology , in vitro , andrology , gene , genetics , medicine
Bovine primary uterine endometrial epithelial cells ( EECs ) are not ideal for long‐term studies, because primary EECs lose hormone responsiveness quickly, and/or they tend to have a short life span. The aims of this study were to establish immortalized bovine EECs and to characterize these cells following long‐term cultures. Immortalized bovine EECs were established by transfecting retroviral vectors encoding human papillomavirus ( HPV ) E 6 and E 7 , and human telomerase reverse transcriptase ( hTERT ) genes. Established bovine immortalized EECs ( imEECs ) showed the same morphology as primary EECs , and could be grown without any apparent changes for over 60 passages. In addition, imEECs have maintained the features as EECs , exhibiting oxytocin ( OT ) and interferon tau ( IFNT ) responsiveness. Therefore, these imEECs , even after numbers of passages, could be used as an in vitro model to investigate cellular and molecular mechanisms, by which the uterine epithelium responds to IFNT stimulation, the event required for the maternal recognition of pregnancy in the bovine species.