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Cytokine profiles of phakic and pseudophakic eyes with primary retinal detachment
Author(s) -
Garweg Justus G.,
Zandi Souska,
Pfister Isabel,
Rieben Robert,
Skowronska Magdalena,
Tappeiner Christoph
Publication year - 2019
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/aos.13998
Subject(s) - proliferative vitreoretinopathy , cytokine , retinal detachment , ophthalmology , vitrectomy , medicine , retinal , aqueous humor , immunology , visual acuity
Purpose To compare the cytokine profiles of phakic (p) and pseudophakic (ps) eyes with primary rhegmatogenous retinal detachment ( RD ) to eyes with macular holes ( MH ) and to identify differences in the specific cytokine profiles. Methods Aqueous humour ( AH ) and vitreous fluid ( VF ) were obtained from patients with primary RD without proliferative vitreoretinopathy undergoing vitrectomy. AH and VF of patients with macular holes ( MH ) served as controls. Forty‐three different cytokines were quantified using multiplex cytokine analysis. Intergroup and intragroup comparisons were performed. To control for multiple comparisons, Holm's correction was applied. Results VF and AH samples of 71 eyes with RD ( pRD N = 38; ps RD N = 33) and 26 eyes with MH were included. Cytokine levels in ps RD and pRD were similar (none with >10‐fold difference). The levels of 39 of 43 cytokines in the VF were significantly higher in eyes with RD than in those with MH (>10‐fold: CXLC 5, CCL 26, CCL 1, IL ‐6, CXCL 11, CCL 7, CCL 13, MIG / CXCL 9, CCL 19 and TGF ‐ β 1). In the AH , 23 of 43 cytokines were significantly higher compared to MH (>10‐fold: CXCL 5, IL ‐4, IL ‐6, IL ‐8/ CXCL 8 and CCL 7). Conclusion A complex, but nonspecific cytokine environmental response seems to initiate immunological and profibrotic processes following RD . Relevant differences in the cytokine profiles of eyes with pRD and ps RD were not identified, whereas cytokine differences between AH and VF in RD could be explained by upregulation in the vitreous, a higher turn around in the anterior chamber, or differences in inflammatory cascades in both compartments.