Premium
Screening of Living Kidney Donors for Genetic Diseases Using a Comprehensive Genetic Testing Strategy
Author(s) -
Thomas C. P.,
Mansilla M. A.,
Sompallae R.,
Mason S. O.,
Nishimura C. J.,
Kimble M. J.,
Campbell C. A.,
Kwitek A. E.,
Darbro B. W.,
Stewart Z. A.,
Smith R. J. H.
Publication year - 2017
Publication title -
american journal of transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.89
H-Index - 188
eISSN - 1600-6143
pISSN - 1600-6135
DOI - 10.1111/ajt.13970
Subject(s) - medicine , alport syndrome , genetic testing , context (archaeology) , family history , disease , genetic counseling , autosomal dominant polycystic kidney disease , genetics , kidney disease , kidney transplantation , kidney , glomerulonephritis , biology , paleontology
Related living kidney donors ( LKD s) are at higher risk of end‐stage renal disease ( ESRD ) compared with unrelated LKD s. A genetic panel was developed to screen 115 genes associated with renal diseases. We used this panel to screen six negative controls, four transplant candidates with presumed genetic renal disease and six related LKD s. After removing common variants, pathogenicity was predicted using six algorithms to score genetic variants based on conservation and function. All variants were evaluated in the context of patient phenotype and clinical data. We identified causal variants in three of the four transplant candidates. Two patients with a family history of autosomal dominant polycystic kidney disease segregated variants in PKD 1 . These findings excluded genetic risk in three of four relatives accepted as potential LKD s. A third patient with an atypical history for Alport syndrome had a splice site mutation in COL 4A5 . This pathogenic variant was excluded in a sibling accepted as an LKD . In another patient with a strong family history of ESRD , a negative genetic screen combined with negative comparative genomic hybridization in the recipient facilitated counseling of the related donor. This genetic renal disease panel will allow rapid, efficient and cost‐effective evaluation of related LKD s.