Open AccessExopolysaccharides from a Codonopsis pilosula endophyte activate macrophages and inhibit cancer cell proliferation and migration
Author(s) -
Chen Min,
Li Yuanyuan,
Liu Zhu,
Qu Yajun,
Zhang Huajie,
Li Dengwen,
Zhou Jun,
Xie Songbo,
Liu Min
Publication year - 2018
Publication title -
thoracic cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 28
eISSN - 1759-7714
pISSN - 1759-7706
DOI - 10.1111/1759-7714.12630
Subject(s) - flow cytometry , immunofluorescence , cancer cell , apoptosis , cell , medicine , microbiology and biotechnology , cancer , biochemistry , biology , antibody , immunology
Background Exopolysaccharides with structural diversity have shown wide applications in biomaterial, food, and pharmaceutical industries. Herein, we isolated an endophytic strain, 14‐DS‐1, from the traditional medicinal plant Codonopsis pilosula to elucidate the characteristics and anti‐cancer activities of purified exopolysaccharides. Methods HPLC and GC‐MS were conducted to purify and characterize the exopolysaccharides isolated from 14‐DS‐1. Quantitative RT‐PCR, cell migration assays, immunofluorescence staining, and flow cytometry analysis were conducted to investighate the biological activity of DSPS. Results We demonstrated that exopolysaccharides isolated from 14‐DS‐1 (DSPS), which were predominately composed of six monosaccharides, showed anti‐cancer activities. Biological activity analysis revealed that exposure to DSPS induced macrophage activation and polarization by promoting the production of TNF‐α and nitric oxide. Further analysis revealed that DSPS treatment promoted macrophage infiltration, whereas cancer cell migration was suppressed. In addition, DSPS exposure led to S‐phase arrest and apoptosis in cancer cells. Immunofluorescence staining revealed that treatment with DSPS resulted in defects in spindle orientation and positioning. Conclusion These findings thus suggest that DSPS may have promising potential in cancer therapy.