Open AccessEpac1, PDE 4, and PKC protein expression and their association with AKAP 95, C x43, and cyclin D 2/ E 1 in breast cancer tissues
Author(s) -
Huang Ping,
Sun Qian,
Zhuang Wenxin,
Peng Kuan,
Wang Dai,
Yao Youliang,
Guo Dongbei,
Zhang Lu,
Shen Chuhan,
Sun Mengyun,
Tang Chaoying,
Teng Bogang,
Zhang Yongxing
Publication year - 2017
Publication title -
thoracic cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 28
eISSN - 1759-7714
pISSN - 1759-7706
DOI - 10.1111/1759-7714.12475
Subject(s) - cyclin d1 , cyclin , breast cancer , protein kinase c , cyclin d , medicine , breast carcinoma , cancer research , microbiology and biotechnology , cancer , kinase , biology , cell cycle
Background This study was conducted to investigate the exchange protein directly activated by c AMP ( E pac1), PDE 4, and PKC expression in breast cancer tissues, and the correlation between these proteins and AKAP 95, C x43, cyclin D 2, and cyclin E 1. Methods PV ‐9000 two‐step immunohistochemistry was used to analyze protein expression. Results The positive rate of E pac1 protein expression in breast cancer tissues (58%) was higher than in para‐carcinoma tissues (10%) ( P < 0.05). There were no significant differences in the positive rates of PDE 4 and PKC expression between breast cancer and para‐carcinoma tissues ( P > 0.05). The positive expression rate of PDE 4 was higher in the P 53 protein positive group compared to the P 53 negative group ( P < 0.05). Correlations between E pac1 and cyclin D 2, PDE 4 and cyclin D 2, AKAP 95 and PKC , C x43 and PKC, and cyclin D 2 and PKC proteins were observed ( P < 0.05). Conclusion E pac1 expression in breast cancer tissues was increased, suggesting that the protein may be involved in the development of breast cancer. Correlations between E pac1 and cyclin D 2, PDE 4 and cyclin D 2, AKAP 95 and PKC , C x43 and PKC , and cyclin D 2 and PKC proteins suggested synergistic effects among these proteins in the development of breast cancer.