Open AccessExpression of P ax8 is decreased and bortezomib does not increase the iodine uptake in thyroid carcinoma cells
Author(s) -
Wang Huanjun,
Yao Jinming,
Zhang Zhongwen,
Zhao Junyu,
Shang Hongxia,
Liao Lin,
Dong Jianjun
Publication year - 2015
Publication title -
thoracic cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 28
eISSN - 1759-7714
pISSN - 1759-7706
DOI - 10.1111/1759-7714.12277
Subject(s) - bortezomib , thyroid carcinoma , medicine , thyroid cancer , western blot , cancer research , proteasome inhibitor , thyroid , papillary thyroid cancer , iodine , carcinoma , thyroidectomy , pax8 , endocrinology , multiple myeloma , biology , chemistry , biochemistry , transcription factor , gene , organic chemistry
Fundamental treatment for papillary thyroid carcinoma ( PTC ) involves total or subtotal thyroidectomy. Iodine‐131 ( 131 I ) is routinely utilized to target remnant thyroid cancer and metastasis after thyroidectomy. The effectiveness of other therapeutic modalities remains unsatisfactory; thus, these patients have a poor prognosis. The manner in which the ability of 131 I uptake can be improved is vital for their prognosis. Bortezomib has been used as a re‐differentiation agent for the treatment of patients with multiple myeloma; however, little is reported about the role of bortezomib in thyroid cancer. To evaluate the therapeutic potential of bortezomib in a human PTC cell line, expression of paired‐box 8 ( P ax8) protein was determined using W estern blot in PTC , normal thyroid, and anaplastic/undifferentiated thyroid carcinoma ( ATC ) cells. The expression of P ax8 protein in PTC cells pretreated with bortezomib was determined using the same method. Iodine uptake was determined using 131 I radioactivity assay. The level of P ax8 protein in normal thyroid cells was significantly higher than in PTC ( P < 0.05) and ATC cells ( P < 0.05); its expression in PTC cells was also significantly higher than in ATC cells ( P < 0.05). The PTC cells in the bortezomib‐treated group showed a higher expression of P ax8 protein than the control group ( P < 0.05). These findings indicate that bortezomib can increase the expression of P ax8, but does not significantly increase the iodine uptake of PTC cells.